anti nkg2a antibody  (Bioss)

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: Alignment of the NKG2A VHH sequence with the reference sequence from GenBank. The complementarity-determining region 3 (CDR3) motif is highlighted with yellow boxes.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: Sequencing

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: Expression of VHH in NKG2A-VHH-A3-1 at varying concentrations of IPTG, as analyzed by western blotting.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: Expressing, Western Blot

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: Expression levels of VHH in NKG2A-VHH-A3-1 at various IPTG concentrations, assessed by Coomassie Blue staining.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: Expressing, Staining

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: (A) SDS-PAGE (heated CBB staining) results and (B) Western blotting results showed the results of NKG2A VHH affinity chromatography.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: SDS Page, Staining, Western Blot, Affinity Chromatography

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: The affinity between NKG2A VHH and the antigen was detected using indirect ELISA. An anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS acted as a negative control instead of the NKG2A polypeptide antigen. BSA was used as a blank control. Three replicates were established for each group. *** p < 0.001.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: Indirect ELISA, Positive Control, Negative Control, Control

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: NKG2A VHH was used to assess the distribution of NKG2A in tissues. PBS served as a negative control. The NKG2A VHH is located in NK cells in the red pulp region of spleen tissue. Red arrows indicate the red pulp region, while the black arrow represents the zoom.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: Negative Control

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: The effect of NKG2A VHH nanobody at different doses on melanoma growth in the Balb/c mouse model. (A) Schematic diagram of NKG2A VHH for the treatment of malignant melanoma in mice. (B) Compared to the PBS control group, the 30 μg dose had a minimal effect, the 50 μg dose showed a noticeable effect, and the 70 μg dose demonstrated a significant effect. (C) Tumor weights in the control group were compared to the experimental group after drug treatment. *** p < 0.001. (D) Growth rates of allogeneic tumors in Balb/c mice at various doses during tumor volume assays from 0 to 30 days.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques: Control

Journal: Frontiers in Veterinary Science

Article Title: A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma

doi: 10.3389/fvets.2025.1571857

Figure Lengend Snippet: Model for the preparation of novel nanoantibody drugs for NKG2A VHH and treatment for MM.

Article Snippet: Anti-NKG2A antibody (Bioss, bs-2411R) served as a positive control, while PBS was used as a negative control instead of the NKG2A polypeptide antigen.

Techniques:

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: A ) Body weight was measured chronologically. B ) The frequency of NKG2A+ T cells in PBMCs was analyzed by flow cytometry. Data were calculated as the ratio of NKG2A+CD3+ cells to CD3+ cells. ▪, DSS-induced colitis mice; ▵, control mice. For both DSS-induced colitis and control mice, 3 to 10 mice were subjected to analysis at each time point. *P<0.05.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Flow Cytometry, Control

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: A ) Frequency of NKG2A+ T cells in PBMCs of DSS-induced colitis mice. B ) Frequency of NKG2A+ T cells in LPMCs of DSS-induced colitis mice. Data are expressed as the ratio of NKG2A+CD3+ cells to CD3+ cells. For each time point, 8 or 9 mice were subjected to analysis. *P<0.05.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques:

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: Frequencies of CD8+ T cells in CD3+ LPMCs (A), TCRγδ+ T cells in CD3+ LPMCs (B), CD49b+ T cells in CD3+ LMPCs (C), NKG2A+ cells in CD3+CD8+ LPMCs (D), NKG2A+ cells in CD3+TCRγδ+ LPMCs (E), and NKG2A+ cells in CD3+CD49b+ LPMCs (F) were analyzed by flow cytometry. For each time point, 4 to 7 mice were subjected to analysis. *P<0.05.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: For Ab blocking experiments, mice were intraperitoneally injected with 300 µg of anti-NKG2A monoclonal Ab or control IgG. The number of inflammatory foci in the intestine was counted histologically on day 6 after DSS administration. Seven mice injected with anti-NKG2D Ab and 8 mice injected with control IgG were subjected to analysis.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Blocking Assay, Injection, Control

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: A ) Representative flow cytometry analysis of peripheral blood NKG2A+ T cells in HC and IBD patients. B ) The ratio of NKG2A+CD3+ cells to CD3+ PBMCs was analyzed by flow cytometry. PBMCs from 23 HCs, 20 patients with UC, and 16 patients with CD were subjected to analysis. Numbers in parentheses represent mean ± standard deviation. *P<0.05.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Flow Cytometry, Standard Deviation

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: Data are expressed as the ratio of NKG2A+CD3+ cells to CD3+ cells. Clinical data are summarized in . Numbers in parentheses represent mean ± standard deviation.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: A ) Ratio of NKG2A+ cells in CD3+CD8+ T cells, B ) Ratio of NKG2A+ cells in CD3+CD56+ NKT cells, C ) Ratio of NKG2A+ cells in CD3+TCRγδ+ T cells, and D ) Ratio of NKG2A+ cells in CD3-CD56+ NK cells. Numbers in parentheses represent mean ± standard deviation. PBMCs isolated from 23 HCs, 20 patients with UC, and 16 patients with CD were subjected to analysis. *P<0.05.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Standard Deviation, Isolation

Journal: PLoS ONE

Article Title: Decrease of Peripheral and Intestinal NKG2A-Positive T Cells in Patients with Ulcerative Colitis

doi: 10.1371/journal.pone.0044113

Figure Lengend Snippet: A ) A representative double-stained section of normal intestinal tissues using anti-CD3 and anti-NKG2A Abs. Merged images show double positive cells (arrow). Red, CD3; green, NKG2A. B ) The frequency of NKG2A+ T cells in the intestine was analyzed by immunohistochemistry. NKG2A+ T cells in the lamina propria were less abundant in UC patients than in HC and CD patients. Tissue sections from 7 HCs, 6 UC patients, and 5 CD patients were subjected to analysis. *P<0.05.

Article Snippet: The slides were incubated in PBS with 10% goat serum for 1 hour at room temperature, followed by overnight incubation with goat polyclonal Ab against human NKG2A (1∶100, T-20, Santa Cruz) at 4°C.

Techniques: Staining, Immunohistochemistry